Kinetic Model of Electron-Transport Reactions in Thylakoid Membranes Determining Chlorophyll Fluorescence Transients
نویسندگان
چکیده
منابع مشابه
Valinomycin sensitivity proves that light-induced thylakoid voltages result in millisecond phase of chlorophyll fluorescence transients.
Upon sudden exposure of plants to an actinic light of saturating intensity, the yield of chlorophyll fluorescence increases typically by 200-400% of the initial O-level. At least three distinct phases of these O-J-I-P transients can be resolved: O-J (0.05-5 ms), J-I (5-50 ms), and I-P (50-1000 ms). In thylakoid membranes, the J-I increase accounts for approximately 30% of the total fluorescence...
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We present here a comparative study on the decay of chlorophyll (Chl) a fluorescence yield in thylakoid membranes and photosystem II (PSII)-enriched samples, measured with multifrequency cross-correlation phase fluorometry. These measurements confirm the general conclusions of Van Mieghem et al. (Biochim. Biophys. Acta 1100, 198-206, 1992), obtained with a flash method, on the effects of reduct...
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Upon illumination, a dark-adapted photosynthetic sample shows time-dependent changes in chlorophyll (Chl) a fluorescence yield, known as the Kautsky phenomenon or the OIDPS transient. Based on the differential effects of electron acceptors such as 2,5-dimethyl-p-benzoquinone (DMQ) and 2,6-dichloro-p-benzoquinone (DCBQ) on Chl a fluorescence transients of spinach thylakoids, we suggest that the ...
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For more than half a century, electron microscopy has been a main tool for investigating the complex ultrastructure and organization of chloroplast thylakoid membranes, but, even today, the three-dimensional relationship between stroma and grana thylakoids, and the arrangement of the membrane protein complexes within them are not fully understood. Electron cryo-tomography (cryo-ET) is a powerfu...
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The initial rate of Cu2+ movement across the thylakoid membrane of pea (Pisum sativum) chloroplasts was directly measured by stopped-flow spectrofluorometry using membranes loaded with the Cu(2+)-sensitive fluorophore Phen Green SK. Cu2+ transport was rapid, reaching completion within 0.5 s. The initial rate of uptake was dependent upon Cu2+ concentration and saturated at about 0.6 microm total...
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ژورنال
عنوان ژورنال: Biotechnology & Biotechnological Equipment
سال: 2009
ISSN: 1310-2818,1314-3530
DOI: 10.1080/13102818.2009.10818501